Gene Autry is back near the saddle, trying to help out a crippled jockey. Gene is certain that the jockey can ride in the Big Race if the lad can regain his self-confidence. Meanwhile, Gene and comical sidekick Sterling Holloway have another problem on their hands: A rogue stallion has "kidnapped" Gene's prize mare. Piloting a plane, Autry seeks out and locates the stallion. The jockey and mare enter the race, and win. Director John English keeps Trail to San Antone constantly on the move, resulting in one of the better postwar Gene Autry vehicles for Republic Studios.